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535 questions in this collection · Page 24 of 54
Question 231 of 535
📘 CLASS XII
Biotechnology Principles And Processes
If foreign DNA is ligated at the BamH I site in the vector pBR322, the BamH I site is located in the gene for resistance to:
Foreign DNA can be ligated at the BamH I site of tetracycline resistance gene (*tetR*) in the vector pBR322.
Question 232 of 535
📘 CLASS XII
Biotechnology Principles And Processes
Insertion of foreign DNA into the tetracycline resistance gene (*tetR*) of pBR322 causes the recombinant plasmids to lose tetracycline resistance. This phenomenon is called:
The recombinant plasmids will lose tetracycline resistance due to insertion of foreign DNA, which is referred to as insertional inactivation.
Question 233 of 535
📘 CLASS XII
Biotechnology Principles And Processes
Why is the selection of recombinants using antibiotic resistance genes considered a cumbersome procedure?
Selection of recombinants due to inactivation of antibiotics is cumbersome because it requires simultaneous plating on two plates.
Question 234 of 535
📘 CLASS XII
Biotechnology Principles And Processes
Alternative selectable markers differentiate recombinants from non-recombinants primarily based on their ability to produce:
Alternative selectable markers differentiate recombinants on the basis of their ability to produce colour in the presence of a chromogenic substrate.
Question 235 of 535
📘 CLASS XII
Biotechnology Principles And Processes
In chromogenic selection, the recombinant DNA is inserted within the coding sequence of which enzyme's gene?
A recombinant DNA is inserted within the coding sequence of the enzyme $\beta$-galactosidase.
Question 236 of 535
📘 CLASS XII
Biotechnology Principles And Processes
If a plasmid does not have an insert (non-recombinant) during chromogenic selection, the colonies appear:
The presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert.
Question 237 of 535
📘 CLASS XII
Biotechnology Principles And Processes
Recombinant colonies in chromogenic selection are identified because they do not produce any colour, often appearing colourless or white, due to:
Recombinant colonies do not produce any colour due to insertional inactivation of the $\beta$-galactosidase gene.
Question 238 of 535
📘 CLASS XII
Biotechnology Principles And Processes
Which bacterium, a pathogen of several dicot plants, is used as a source for plant gene transfer vectors?
*Agrobacterium tumifaciens* is a pathogen of several dicot plants used for transferring genes.
Question 239 of 535
📘 CLASS XII
Biotechnology Principles And Processes
*Agrobacterium tumifaciens* delivers a piece of DNA known as ‘T-DNA’ to plant cells, resulting in the formation of a:
It delivers a piece of DNA known as ‘T-DNA’ to transform normal plant cells into a tumor.
Question 240 of 535
📘 CLASS XII
Biotechnology Principles And Processes
In animals, which viral structures have the ability to transform normal cells into cancerous cells and have been disarmed for use as gene delivery vectors?
Retroviruses in animals have the ability to transform normal cells into cancerous cells, and have been disarmed for use as vectors.