Class XII · Biology practice

Biotechnology Principles and Processes MCQs

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535 questions in this collection · Page 25 of 54

Question 241 of 535

📘 CLASS XII

Biotechnology Principles And Processes

The modified cloning vector derived from *Agrobacterium tumifaciens* that is utilized to deliver genes of interest into a variety of plants is the:

The tumor inducing (Ti) plasmid has been modified into a cloning vector which is no more pathogenic.

Question 242 of 535

📘 CLASS XII

Biotechnology Principles And Processes

DNA cannot easily pass through cell membranes because it is a _________ molecule.

Since DNA is a hydrophilic molecule, it cannot pass through cell membranes.

Question 243 of 535

📘 CLASS XII

Biotechnology Principles And Processes

To make bacterial cells competent to take up DNA, they are treated with a specific concentration of a divalent cation, typically:

Bacterial cells are treated with a specific concentration of a divalent cation, such as calcium.

Question 244 of 535

📘 CLASS XII

Biotechnology Principles And Processes

What is the crucial temperature used during the brief heat shock step to enable competent bacteria to take up recombinant DNA?

Cells are briefly placed at 42°C (heat shock) to force the bacteria to take up the recombinant DNA.

Question 245 of 535

📘 CLASS XII

Biotechnology Principles And Processes

In which method is recombinant DNA directly injected into the nucleus of an animal cell?

Micro-injection involves directly injecting recombinant DNA into the nucleus of an animal cell.

Question 246 of 535

📘 CLASS XII

Biotechnology Principles And Processes

The method suitable for plants where cells are bombarded with high velocity micro-particles coated with DNA is known as:

Cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA in a method known as biolistics or gene gun.

Question 247 of 535

📘 CLASS XII

Biotechnology Principles And Processes

The first step in the specific sequence of Recombinant DNA Technology processes is the:

Recombinant DNA technology involves several steps, the first of which is isolation of DNA.

Question 248 of 535

📘 CLASS XII

Biotechnology Principles And Processes

To break open bacterial cells and release DNA, the enzyme used is:

Bacterial cells are treated with enzymes such as lysozyme.

Question 249 of 535

📘 CLASS XII

Biotechnology Principles And Processes

To break open plant cells and release DNA, the enzyme used is:

Plant cells are treated with enzymes such as cellulase.

Question 250 of 535

📘 CLASS XII

Biotechnology Principles And Processes

To break open fungal cells and release DNA, the enzyme used is:

Fungal cells are treated with enzymes such as chitinase.

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