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Biotechnology Principles and Processes MCQs
Class XII Biology
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NCERT Based
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NEET Practice
📘 Concept Based
📝 Exam Level
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535 questions in this chapter
Question 241 of 535
📘 CLASS XII
The modified cloning vector derived from *Agrobacterium tumifaciens* that is utilized to deliver genes of interest into a variety of plants is the:
The tumor inducing (Ti) plasmid has been modified into a cloning vector which is no more pathogenic.
Question 242 of 535
📘 CLASS XII
DNA cannot easily pass through cell membranes because it is a _________ molecule.
Since DNA is a hydrophilic molecule, it cannot pass through cell membranes.
Question 243 of 535
📘 CLASS XII
To make bacterial cells competent to take up DNA, they are treated with a specific concentration of a divalent cation, typically:
Bacterial cells are treated with a specific concentration of a divalent cation, such as calcium.
Question 244 of 535
📘 CLASS XII
What is the crucial temperature used during the brief heat shock step to enable competent bacteria to take up recombinant DNA?
Cells are briefly placed at 42°C (heat shock) to force the bacteria to take up the recombinant DNA.
Question 245 of 535
📘 CLASS XII
In which method is recombinant DNA directly injected into the nucleus of an animal cell?
Micro-injection involves directly injecting recombinant DNA into the nucleus of an animal cell.
Question 246 of 535
📘 CLASS XII
The method suitable for plants where cells are bombarded with high velocity micro-particles coated with DNA is known as:
Cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA in a method known as biolistics or gene gun.
Question 247 of 535
📘 CLASS XII
The first step in the specific sequence of Recombinant DNA Technology processes is the:
Recombinant DNA technology involves several steps, the first of which is isolation of DNA.
Question 248 of 535
📘 CLASS XII
To break open bacterial cells and release DNA, the enzyme used is:
Bacterial cells are treated with enzymes such as lysozyme.
Question 249 of 535
📘 CLASS XII
To break open plant cells and release DNA, the enzyme used is:
Plant cells are treated with enzymes such as cellulase.
Question 250 of 535
📘 CLASS XII
To break open fungal cells and release DNA, the enzyme used is:
Fungal cells are treated with enzymes such as chitinase.
