The thermostable DNA polymerase remains active during the high temperature step in PCR, which is required for:
The enzyme remains active during the high temperature induced denaturation of double stranded DNA.
Question 262 of 535
📘 CLASS XII
If transformed *E. coli* cells (carrying ampicillin resistance) are spread on agar plates containing ampicillin, what happens to the untransformed recipient cells?
Untransformed recipient cells will die because they lack the resistance gene.
Question 263 of 535
📘 CLASS XII
The ampicillin resistance gene in a recombinant DNA molecule is referred to as a selectable marker because it allows the researcher to:
The ampicillin resistance gene is called a selectable marker because one is able to select a transformed cell in its presence.
Question 264 of 535
📘 CLASS XII
If a protein encoding gene is expressed in a heterologous host (an alien host), the resulting protein is called a:
If any protein encoding gene is expressed in a heterologous host, it is called a recombinant protein.
Question 265 of 535
📘 CLASS XII
In a continuous culture system, cells are maintained in their physiologically most active phase to produce a larger biomass. This phase is the:
Continuous culturing maintains cells in their physiologically most active log/exponential phase for higher yields of desired protein.
Question 266 of 535
📘 CLASS XII
Bioreactors were developed to process large volumes of culture, typically between:
Bioreactors process large volumes (100-1000 litres) of culture.
Question 267 of 535
📘 CLASS XII
A bioreactor provides optimal growth conditions by controlling temperature, pH, substrate, salts, vitamins, and:
Optimal growth conditions provided by a bioreactor include oxygen availability.
Question 268 of 535
📘 CLASS XII
The most commonly used bioreactors are of the:
The most commonly used bioreactors are of stirring type (simple or sparged).
Question 269 of 535
📘 CLASS XII
What feature of a stirred-tank bioreactor facilitates even mixing and oxygen availability throughout the vessel?
The stirrer facilitates even mixing and oxygen availability throughout the bioreactor.
Question 270 of 535
📘 CLASS XII
After the biosynthetic stage, the processes including separation and purification are collectively referred to as:
Separation and purification processes are collectively referred to as downstream processing.
