RNA can be removed from the DNA preparation by treatment with the enzyme:

[Biotechnology-Principles-and-Processes] [class-xii ]

• Protease
• Ribonuclease
• Deoxyribonuclease
• Ligase
Correct Option: B  [ Ribonuclease ]

Remark: The RNA can be removed by treatment with ribonuclease.

Proteins can be removed from the DNA preparation by treatment with the enzyme:

[Biotechnology-Principles-and-Processes] [class-xii ]

• Ribonuclease
• Cellulase
• Protease
• Chitinase
Correct Option: C  [ Protease ]

Remark: Proteins can be removed by treatment with protease.

Purified DNA ultimately precipitates out of the suspension after the addition of chilled:

[Biotechnology-Principles-and-Processes] [class-xii ]

• Water
• Glycerol
• Ethanol
• Acetone
Correct Option: C  [ Ethanol ]

Remark: Purified DNA ultimately precipitates out after the addition of chilled ethanol.

The technique used to remove the precipitated DNA, visible as fine threads in suspension, is known as:

[Biotechnology-Principles-and-Processes] [class-xii ]

• Elution
• Spooling
• Electrophoresis
• Ligation
Correct Option: B  [ Spooling ]

Remark: The precipitated DNA can be removed by spooling.

Agarose gel electrophoresis is employed to check the progression of which key RDT step?

[Biotechnology-Principles-and-Processes] [class-xii ]

• DNA ligation
• Primer annealing
• Restriction enzyme digestion
• DNA purification
Correct Option: C  [ Restriction enzyme digestion ]

Remark: Agarose gel electrophoresis is employed to check the progression of a restriction enzyme digestion.

After cutting the source DNA and vector DNA, which enzyme is added to mix the cut components and prepare recombinant DNA?

[Biotechnology-Principles-and-Processes] [class-xii ]

• DNA polymerase
• DNA ligase
• Restriction endonuclease
• Exonuclease
Correct Option: B  [ DNA ligase ]

Remark: Ligase is added to join the cut source DNA and the cut vector, resulting in the preparation of recombinant DNA.

What is the full form of PCR?

[Biotechnology-Principles-and-Processes] [class-xii ]

• Primary Chain Replication
• Polymerase Chain Reaction
• Protein Counting Ratio
• Palindromic Cloning Routine
Correct Option: B  [ Polymerase Chain Reaction ]

Remark: PCR stands for Polymerase Chain Reaction.

Multiple copies of the gene of interest are synthesized *in vitro* during PCR using two sets of primers and the enzyme:

[Biotechnology-Principles-and-Processes] [class-xii ]

• DNA ligase
• Restriction enzyme
• DNA polymerase
• RNA transcriptase
Correct Option: C  [ DNA polymerase ]

Remark: DNA polymerase is the enzyme used in PCR.

The segment of DNA can be amplified to approximately how many copies if the replication process in PCR is repeated many times?

[Biotechnology-Principles-and-Processes] [class-xii ]

• Thousand times
• Million times
• Billion times
• Trillion times
Correct Option: C  [ Billion times ]

Remark: The segment of DNA can be amplified to approximately billion times, i.e., 1 billion copies are made.

The thermostable DNA polymerase used in PCR is isolated from the bacterium:

[Biotechnology-Principles-and-Processes] [class-xii ]

• Escherichia coli
• Salmonella typhimurium
• Agrobacterium tumifaciens
• Thermus aquaticus
Correct Option: D  [ Thermus aquaticus ]

Remark: The thermostable DNA polymerase is isolated from a bacterium, *Thermus aquaticus*.