Class XII · Biology practice

Biotechnology Principles and Processes MCQs

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535 questions in this collection · Page 23 of 54

Question 221 of 535

📘 CLASS XII

Biotechnology Principles And Processes

Which compound is used to stain DNA fragments for visualization in gel electrophoresis?

DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide.

Question 222 of 535

📘 CLASS XII

Biotechnology Principles And Processes

Ethidium bromide stained DNA bands appear bright orange when exposed to:

Bright orange coloured bands are seen when the stained gel is exposed to UV light.

Question 223 of 535

📘 CLASS XII

Biotechnology Principles And Processes

The step of cutting out the separated bands of DNA from the agarose gel and extracting the DNA fragments is known as:

This step of extracting purified DNA fragments from the gel piece is known as elution.

Question 224 of 535

📘 CLASS XII

Biotechnology Principles And Processes

Plasmids and bacteriophages used as cloning vectors replicate within bacterial cells:

Plasmids and bacteriophages have the ability to replicate within bacterial cells independent of the control of chromosomal DNA.

Question 225 of 535

📘 CLASS XII

Biotechnology Principles And Processes

Which feature of bacteriophages makes them suitable vectors for achieving very high copy numbers of a linked alien DNA?

Bacteriophages, because of their high number per cell, have very high copy numbers of their genome.

Question 226 of 535

📘 CLASS XII

Biotechnology Principles And Processes

The vector sequence responsible for initiating replication and controlling the copy number of the linked DNA is the:

The ori sequence is responsible for initiating replication and controlling the copy number of the linked DNA.

Question 227 of 535

📘 CLASS XII

Biotechnology Principles And Processes

A selectable marker in a vector serves the function of identifying and eliminating:

A selectable marker helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.

Question 228 of 535

📘 CLASS XII

Biotechnology Principles And Processes

Transformation is the procedure through which:

Transformation is a procedure through which a piece of DNA is introduced in a host bacterium.

Question 229 of 535

📘 CLASS XII

Biotechnology Principles And Processes

Besides ampicillin, chloramphenicol, and tetracycline, which antibiotic resistance gene is listed as a useful selectable marker for *E. coli*?

Genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, are useful selectable markers for E. coli.

Question 230 of 535

📘 CLASS XII

Biotechnology Principles And Processes

Ideally, a cloning vector needs to have very few, preferably single, recognition sites for commonly used restriction enzymes because:

Presence of more than one recognition site within the vector will generate several fragments, which will complicate the gene cloning.

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