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Biotechnology Principles and Processes MCQs
Class XII Biology
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NCERT Based
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NEET Practice
📘 Concept Based
📝 Exam Level
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535 questions in this chapter
Question 221 of 535
📘 CLASS XII
Which compound is used to stain DNA fragments for visualization in gel electrophoresis?
DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide.
Question 222 of 535
📘 CLASS XII
Ethidium bromide stained DNA bands appear bright orange when exposed to:
Bright orange coloured bands are seen when the stained gel is exposed to UV light.
Question 223 of 535
📘 CLASS XII
The step of cutting out the separated bands of DNA from the agarose gel and extracting the DNA fragments is known as:
This step of extracting purified DNA fragments from the gel piece is known as elution.
Question 224 of 535
📘 CLASS XII
Plasmids and bacteriophages used as cloning vectors replicate within bacterial cells:
Plasmids and bacteriophages have the ability to replicate within bacterial cells independent of the control of chromosomal DNA.
Question 225 of 535
📘 CLASS XII
Which feature of bacteriophages makes them suitable vectors for achieving very high copy numbers of a linked alien DNA?
Bacteriophages, because of their high number per cell, have very high copy numbers of their genome.
Question 226 of 535
📘 CLASS XII
The vector sequence responsible for initiating replication and controlling the copy number of the linked DNA is the:
The ori sequence is responsible for initiating replication and controlling the copy number of the linked DNA.
Question 227 of 535
📘 CLASS XII
A selectable marker in a vector serves the function of identifying and eliminating:
A selectable marker helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.
Question 228 of 535
📘 CLASS XII
Transformation is the procedure through which:
Transformation is a procedure through which a piece of DNA is introduced in a host bacterium.
Question 229 of 535
📘 CLASS XII
Besides ampicillin, chloramphenicol, and tetracycline, which antibiotic resistance gene is listed as a useful selectable marker for *E. coli*?
Genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, are useful selectable markers for E. coli.
Question 230 of 535
📘 CLASS XII
Ideally, a cloning vector needs to have very few, preferably single, recognition sites for commonly used restriction enzymes because:
Presence of more than one recognition site within the vector will generate several fragments, which will complicate the gene cloning.
