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Biotechnology Principles and Processes MCQs
Class XII Biology
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NCERT Based
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NEET Practice
📘 Concept Based
📝 Exam Level
🤖 AI Explanations
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535 questions in this chapter
Question 191 of 535
📘 CLASS XII
The first recombinant DNA molecule was constructed by linking a gene for antibiotic resistance with a native plasmid of which bacterium?
The construction used a native plasmid of Salmonella typhimurium.
Question 192 of 535
📘 CLASS XII
Stanley Cohen and Herbert Boyer accomplished the construction of the first artificial recombinant DNA molecule in:
Cohen and Boyer accomplished the construction of the first recombinant DNA in 1972.
Question 193 of 535
📘 CLASS XII
The capability of cutting DNA at specific locations became possible with the discovery of the so-called:
Restriction enzymes are known as ‘molecular scissors’.
Question 194 of 535
📘 CLASS XII
Plasmid DNA acts as a _________ to transfer the piece of alien DNA attached to it into the host organism.
Plasmid DNA acts as a vector to transfer the piece of DNA attached to it, similar to how a mosquito transfers a malarial parasite.
Question 195 of 535
📘 CLASS XII
Which enzyme was necessary to link the antibiotic resistance gene with the plasmid vector by joining the cut DNA ends?
DNA ligase acts on cut DNA molecules and joins their ends, enabling the linking of the gene with the vector.
Question 196 of 535
📘 CLASS XII
A new combination of circular autonomously replicating DNA created *in vitro* by joining cut DNA molecules is known as:
This new combination, created in vitro, is known as recombinant DNA.
Question 197 of 535
📘 CLASS XII
What is the first basic step in genetically modifying an organism?
The first basic step is the identification of DNA with desirable genes.
Question 198 of 535
📘 CLASS XII
The final basic step in genetically modifying an organism is maintenance of introduced DNA in the host and transfer of the DNA to its _________.
The introduced DNA must be maintained in the host and transferred to its progeny.
Question 199 of 535
📘 CLASS XII
Besides restriction enzymes, polymerase enzymes, and ligases, the key tools of recombinant DNA technology include vectors and the:
The key tools are restriction enzymes, polymerase enzymes, ligases, vectors and the host organism.
Question 200 of 535
📘 CLASS XII
In 1963, two enzymes were isolated responsible for restricting bacteriophage growth in *E. coli*. One cut DNA, and the other:
One of these added methyl groups to DNA, while the other cut DNA (restriction endonuclease).
