Question 211 of 535
Endonucleases function by making cuts at:
Endonucleases make cuts at specific positions within the DNA.
Biotechnology Principles and Processes MCQs
Class XII Biology • NCERT Based • NEET Practice
Question 212 of 535
A restriction endonuclease cuts the DNA double helix at specific points in which part of the strands?
Restriction endonucleases cut each of the two strands of the double helix at specific points in their sugar-phosphate backbones.
Question 213 of 535
Each restriction endonuclease recognizes a specific kind of nucleotide sequence known as a:
Each restriction endonuclease recognises a specific palindromic nucleotide sequence in the DNA.
Question 214 of 535
Restriction enzymes cut the DNA strands away from the center of the palindrome site, leaving overhanging stretches called:
The overhanging stretches are called sticky ends, facilitating joining.
Question 215 of 535
Sticky ends facilitate the action of DNA ligase by forming which type of bonds with their complementary cut counterparts?
Sticky ends form hydrogen bonds with their complementary cut counterparts, which facilitates the action of DNA ligase.
Question 216 of 535
Recombinant DNA molecules are composed of DNA derived from:
Restriction endonucleases are used to form ‘recombinant’ molecules of DNA, which are composed of DNA from different sources/genomes.
Question 217 of 535
The technique used to separate DNA fragments resulting from restriction endonuclease digestion is known as:
The fragments of DNA can be separated by a technique known as gel electrophoresis.
Question 218 of 535
DNA fragments move towards the anode during gel electrophoresis because:
Since DNA fragments are negatively charged molecules they can be separated by forcing them to move towards the anode.
Question 219 of 535
The most commonly used matrix for separating DNA fragments in gel electrophoresis is agarose, which is extracted from:
Agarose is a natural polymer extracted from sea weeds.
Question 220 of 535
In gel electrophoresis, how do DNA fragments separate based on size?
The smaller the fragment size, the farther it moves, due to the sieving effect provided by the agarose gel.