The use of two antibiotic resistance genes in selection is a cumbersome procedure that requires simultaneous plating on _________ plates having different antibiotics.
Selection using antibiotic inactivation requires simultaneous plating on two plates.
Question 62 of 535
📘 CLASS XII
Alternative selectable markers differentiate recombinants based on their ability to produce _________ in the presence of a chromogenic substrate.
Alternative markers use the basis of producing colour.
Question 63 of 535
📘 CLASS XII
In chromogenic selection, insertional inactivation occurs within the coding sequence of the enzyme _________.
The insertion targets the coding sequence of the enzyme $\beta$-galactosidase.
Question 64 of 535
📘 CLASS XII
If a plasmid does not have an insert (non-recombinant), the presence of a chromogenic substrate gives _________ coloured colonies.
Non-recombinant colonies produce blue colour.
Question 65 of 535
📘 CLASS XII
If a plasmid has an insert (recombinant), insertional inactivation occurs, and the colonies do not produce any colour, often appearing _________.
Recombinant colonies do not produce any colour due to insertional inactivation of $\beta$-galactosidase.
Question 66 of 535
📘 CLASS XII
The pathogen of several dicot plants used as a source for gene transfer vectors is _________.
*Agrobacterium tumifaciens* is a pathogen of dicot plants used for transferring genes.
Question 67 of 535
📘 CLASS XII
Agrobacterium tumifaciens delivers a piece of DNA known as _________ to transform normal plant cells into a tumor.
It delivers a piece of DNA known as 'T-DNA'.
Question 68 of 535
📘 CLASS XII
In animals, _________ have the ability to transform normal cells into cancerous cells and have been disarmed for use as vectors.
Retroviruses in animals have been disarmed and are now used to deliver desirable genes.
Question 69 of 535
📘 CLASS XII
The modified cloning vector derived from *Agrobacterium tumifaciens* is the _________ plasmid.
The tumor inducing (Ti) plasmid has been modified into a cloning vector.
Question 70 of 535
📘 CLASS XII
Since DNA is a _________ molecule, it cannot pass through cell membranes easily.
