Plasmid DNA acts as a _________ to transfer a piece of alien DNA attached to it into the host organism.
Plasmid DNA acts as a vector to transfer the piece of DNA attached to it.
Question 22 of 535
📘 CLASS XII
The linking of the antibiotic resistance gene with the plasmid vector was made possible by the enzyme _________.
DNA ligase acts on cut DNA molecules and joins their ends.
Question 23 of 535
📘 CLASS XII
A new combination of circular autonomously replicating DNA created *in vitro* is known as _________.
This new combination, created in vitro, is known as recombinant DNA.
Question 24 of 535
📘 CLASS XII
When recombinant DNA is transferred into *Escherichia coli*, it replicates using the host’s _________ enzyme to make multiple copies.
The transferred DNA replicates using the new host’s DNA polymerase enzyme.
Question 25 of 535
📘 CLASS XII
The first basic step in genetically modifying an organism is the identification of DNA with _________.
The first basic step is the identification of DNA with desirable genes.
Question 26 of 535
📘 CLASS XII
Maintenance of introduced DNA in the host and transfer of the DNA to its _________ is the final basic step in genetically modifying an organism.
The introduced DNA must be maintained and transferred to the progeny.
Question 27 of 535
📘 CLASS XII
Besides restriction enzymes, polymerase enzymes, and ligases, the key tools of recombinant DNA technology include vectors and the _________.
The key tools include restriction enzymes, polymerase enzymes, ligases, vectors and the host organism.
Question 28 of 535
📘 CLASS XII
The two enzymes responsible for restricting the growth of bacteriophage in *Escherichia coli* were isolated in the year _________.
The two restriction enzymes were isolated in 1963.
Question 29 of 535
📘 CLASS XII
The enzyme that cuts DNA, isolated in 1963, was called a restriction _________.
The enzyme that cut DNA was called restriction endonuclease.
Question 30 of 535
📘 CLASS XII
The first restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence, isolated and characterized five years later (1968), was _________.
Hind II was the first restriction endonuclease characterized.
