Choose the correct pair from the following:

NEET-(Sept)2020 [Biotechnology-Principles-and-Processes] [class-xii ]

• Ligases -

  Join the two DNA molecules

• Polymerases -

  Break the DNA into fragments

• Nucleases -

  Separate the two strands of DNA

• Exonucleases -

  Make cuts at specific positions within DNA

Correct Option: A  [
Ligases -

Join the two DNA molecules
]

Remark:

Identify the wrong statement with regard to Restriction Enzymes.

NEET-(Sept)2020 [Biotechnology-Principles-and-Processes] [class-xii ]

• Each restriction enzyme functions by inspecting the length of a DNA sequence.
• They cut the strand of DNA at palindromic sites.
• They are useful in genetic engineering.
• Sticky ends can be joined by using DNA ligases.
Correct Option: D  [ Sticky ends can be joined by using DNA ligases. ]

Remark:

Match the following columns and select the correct option.

NEET-(Sept)2020 [Biotechnology-Principles-and-Processes] [class-xii ]

• (A)-(IV), (B)-(I), (C)-(II), (D)-(III)
• (A)-(III), (B)-(II), (C)-(I), (D)-(IV)
• (A)-(II), (B)-(III), (C)-(IV), (D)-(I)
• (A)-(I), (B)-(II), (C)-(III), (D)-(IV)
Correct Option: A  [ (A)-(IV), (B)-(I), (C)-(II), (D)-(III) ]

Remark:

In gel electrophoresis, separated DNA fragments can be visualized with the help of:

NEET-(Sept)2020 [Biotechnology-Principles-and-Processes] [class-xii ]

• Acetocarmine in bright blue light
• Ethidium bromide in UV radiation
• Acetocarmine in UV radiation
• Ethidium bromide in infrared radiation
Correct Option: D  [ Ethidium bromide in infrared radiation ]

Remark:

Spooling is:

NEET-(Oct)2020 [Biotechnology-Principles-and-Processes] [class-xii ]

• Amplification of DNA
• Cutting of separated DNA bands from the agarose gel
• Transfer of separated DNA fragments to synthetic membranes
• Collection of isolated DNA
Correct Option: D  [ Collection of isolated DNA ]

Remark:

The sequence that controls the copy number of the linked DNA in the vector, is termed:

NEET-(Sept)2020 [Biotechnology-Principles-and-Processes] [class-xii ]

• Selectable marker
• Ori site
• Palindromic sequence
• Recognition site
Correct Option: B  [ Ori site ]

Remark:

Following statements describe the characteristics of the enzyme Restriction endonuclease. Identify the incorrect statement.

NEET-2019 [Biotechnology-Principles-and-Processes] [class-xii ]

• The enzyme binds DNA at specific sites and cuts only one of the two strands.
• The enzyme cuts DNA molecule at identified position within the DNA
• The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
• The enzyme recognizes a specific palindromic nucleotide sequence in the DNA
Correct Option: A  [ The enzyme binds DNA at specific sites and cuts only one of the two strands. ]

Remark:

Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:

NEET-2024 [Biotechnology-Principles-and-Processes] [class-xii ]

• 6 bp
• 8 bp
• 10 bp
• 4 bp
Correct Option: A  [ 6 bp ]

Remark: Explanation: NCERT Page No.195

What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism?

A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism.
B. It may get integrated into the genome of the recipient.
C. It may multiply and be inherited along with the host DNA.
D. The alien piece of DNA is not an integral part of chromosome.
E. It shows ability to replicate.

Choose the correct answer from the options given below:

NEET-2024 [Biotechnology-Principles-and-Processes] [class-xii ]

• D and E only
• A and B only
• A and E only
• B and C only
Correct Option: D  [ B and C only ]

Remark: Explanation: NCERT Page No.194, (Topic-11.1 PRINCIPLES OF BIOTECHNOLOGY)