Class XII · Biology practice

Biotechnology Principles and Processes MCQs

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535 questions in this collection · Page 5 of 54

Question 41 of 535

📘 CLASS XII

Biotechnology Principles And Processes

Each restriction endonuclease recognizes a specific _________ nucleotide sequence in the DNA.

Restriction endonucleases recognize specific palindromic nucleotide sequences.

Question 42 of 535

📘 CLASS XII

Biotechnology Principles And Processes

Restriction enzymes cut the DNA strands away from the center of the palindrome site, leaving overhanging stretches called _________.

The overhanging stretches are called sticky ends.

Question 43 of 535

📘 CLASS XII

Biotechnology Principles And Processes

Sticky ends are named so because they facilitate joining by forming _________ bonds with their complementary cut counterparts.

Sticky ends form hydrogen bonds with their complementary cut counterparts.

Question 44 of 535

📘 CLASS XII

Biotechnology Principles And Processes

Recombinant DNA molecules are composed of DNA from _________ sources/genomes.

Restriction endonucleases are used to form recombinant molecules composed of DNA from different sources/genomes.

Question 45 of 535

📘 CLASS XII

Biotechnology Principles And Processes

The technique used to separate DNA fragments resulting from restriction endonuclease digestion is known as _________.

DNA fragments are separated by gel electrophoresis.

Question 46 of 535

📘 CLASS XII

Biotechnology Principles And Processes

DNA fragments move towards the _________ under an electric field during gel electrophoresis because they are negatively charged molecules.

Since DNA is negatively charged, it moves towards the positive electrode (anode).

Question 47 of 535

📘 CLASS XII

Biotechnology Principles And Processes

The most commonly used matrix for gel electrophoresis is _________, a natural polymer extracted from sea weeds.

Agarose, extracted from sea weeds, is the most commonly used matrix.

Question 48 of 535

📘 CLASS XII

Biotechnology Principles And Processes

In gel electrophoresis, DNA fragments resolve according to their size, meaning the _________ the fragment size, the farther it moves.

Smaller fragments move farther due to the sieving effect of the agarose gel.

Question 49 of 535

📘 CLASS XII

Biotechnology Principles And Processes

Separated DNA fragments are visualized after staining with the compound known as _________.

DNA is stained with ethidium bromide for visualization.

Question 50 of 535

📘 CLASS XII

Biotechnology Principles And Processes

Ethidium bromide stained DNA bands appear bright orange when exposed to _________ radiation.

Bright orange coloured bands are seen when the stained gel is exposed to UV light.

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