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Biotechnology Principles and Processes MCQs
Class XII Biology
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NCERT Based
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NEET Practice
📘 Concept Based
📝 Exam Level
🤖 AI Explanations
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535 questions in this chapter
Question 41 of 535
📘 CLASS XII
Each restriction endonuclease recognizes a specific _________ nucleotide sequence in the DNA.
Restriction endonucleases recognize specific palindromic nucleotide sequences.
Question 42 of 535
📘 CLASS XII
Restriction enzymes cut the DNA strands away from the center of the palindrome site, leaving overhanging stretches called _________.
The overhanging stretches are called sticky ends.
Question 43 of 535
📘 CLASS XII
Sticky ends are named so because they facilitate joining by forming _________ bonds with their complementary cut counterparts.
Sticky ends form hydrogen bonds with their complementary cut counterparts.
Question 44 of 535
📘 CLASS XII
Recombinant DNA molecules are composed of DNA from _________ sources/genomes.
Restriction endonucleases are used to form recombinant molecules composed of DNA from different sources/genomes.
Question 45 of 535
📘 CLASS XII
The technique used to separate DNA fragments resulting from restriction endonuclease digestion is known as _________.
DNA fragments are separated by gel electrophoresis.
Question 46 of 535
📘 CLASS XII
DNA fragments move towards the _________ under an electric field during gel electrophoresis because they are negatively charged molecules.
Since DNA is negatively charged, it moves towards the positive electrode (anode).
Question 47 of 535
📘 CLASS XII
The most commonly used matrix for gel electrophoresis is _________, a natural polymer extracted from sea weeds.
Agarose, extracted from sea weeds, is the most commonly used matrix.
Question 48 of 535
📘 CLASS XII
In gel electrophoresis, DNA fragments resolve according to their size, meaning the _________ the fragment size, the farther it moves.
Smaller fragments move farther due to the sieving effect of the agarose gel.
Question 49 of 535
📘 CLASS XII
Separated DNA fragments are visualized after staining with the compound known as _________.
DNA is stained with ethidium bromide for visualization.
Question 50 of 535
📘 CLASS XII
Ethidium bromide stained DNA bands appear bright orange when exposed to _________ radiation.
Bright orange coloured bands are seen when the stained gel is exposed to UV light.
