The step known as _________ involves cutting out the separated bands of DNA from the agarose gel and extracting the DNA fragments.
Elution is the process of extracting the purified DNA fragments from the gel piece.
Question 52 of 535
📘 CLASS XII
Plasmids and bacteriophages used as cloning vectors have the ability to replicate within bacterial cells _________ of the control of chromosomal DNA.
Plasmids and bacteriophages replicate independent of chromosomal DNA.
Question 53 of 535
📘 CLASS XII
Some plasmids may have 15-100 copies per cell, but bacteriophages typically have very high _________ of their genome within the bacterial cells.
Bacteriophages have very high copy numbers of their genome.
Question 54 of 535
📘 CLASS XII
The sequence in a vector responsible for initiating replication and controlling the copy number of the linked DNA is the _________.
Ori is responsible for initiating replication and controlling the copy number.
Question 55 of 535
📘 CLASS XII
A selectable marker helps in identifying and eliminating _________ and selectively permitting the growth of the transformants.
Selectable markers help in eliminating non-transformants.
Question 56 of 535
📘 CLASS XII
The procedure through which a piece of DNA is introduced into a host bacterium is called _________.
Transformation is the procedure through which DNA is introduced in a host bacterium.
Question 57 of 535
📘 CLASS XII
Genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or _________ are considered useful selectable markers for E. coli.
Kanamycin is listed as a useful selectable marker.
Question 58 of 535
📘 CLASS XII
To facilitate linking alien DNA, a vector needs to have very few, preferably _________, recognition sites for commonly used restriction enzymes.
Presence of more than one site complicates gene cloning, so single sites are preferred.
Question 59 of 535
📘 CLASS XII
The ligation of foreign DNA in the vector pBR322 is often carried out at the BamH I site present in the _________ resistance gene.
Foreign DNA can be ligated at the BamH I site of the tetracycline resistance gene.
Question 60 of 535
📘 CLASS XII
If foreign DNA is inserted into the tetracycline resistance gene of pBR322, the recombinant plasmids will lose tetracycline resistance due to _________.
The loss of function due to insertion of foreign DNA is insertional inactivation.
