Purified DNA ultimately precipitates out of the suspension after the addition of chilled _________.
Chilled ethanol causes purified DNA to precipitate out.
Question 82 of 535
📘 CLASS XII
The precipitated DNA, visible as fine threads in suspension, can be removed by a technique known as _________.
The DNA is removed by spooling.
Question 83 of 535
📘 CLASS XII
Agarose gel electrophoresis is employed to check the progression of a _________ enzyme digestion.
Agarose gel electrophoresis checks the progression of a restriction enzyme digestion.
Question 84 of 535
📘 CLASS XII
The cut gene of interest and the cut vector are mixed, and the enzyme _________ is added to complete the preparation of recombinant DNA.
Ligase joins the cut source DNA and the cut vector.
Question 85 of 535
📘 CLASS XII
PCR stands for _________.
PCR stands for Polymerase Chain Reaction.
Question 86 of 535
📘 CLASS XII
In PCR, multiple copies of the gene of interest are synthesised *in vitro* using two sets of primers and the enzyme _________.
DNA polymerase is the enzyme used in PCR.
Question 87 of 535
📘 CLASS XII
If the replication process in PCR is repeated many times, the segment of DNA can be amplified to approximately _________ times.
The DNA segment can be amplified to approximately billion times (1 billion copies are made).
Question 88 of 535
📘 CLASS XII
The thermostable DNA polymerase used in PCR is isolated from the bacterium _________.
The thermostable DNA polymerase is isolated from *Thermus aquaticus*.
Question 89 of 535
📘 CLASS XII
The thermostable DNA polymerase remains active during the high temperature induced _________ of double-stranded DNA in PCR.
The enzyme remains active during high temperature induced denaturation.
Question 90 of 535
📘 CLASS XII
If a recombinant DNA bearing a gene for resistance to an antibiotic (e.g., ampicillin) is transferred into *E. coli* cells, the host cells become transformed into _________ cells.
The host cells become transformed into ampicillin-resistant cells.
