Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzyme, at the _________ conditions for that specific enzyme.
Digestions are performed at the optimal conditions for that specific enzyme.
Question 112 of 535
📘 CLASS XII
Primers used in PCR are small chemically synthesised _________ that are complementary to the regions of DNA.
Primers are small chemically synthesized oligonucleotides.
Question 113 of 535
📘 CLASS XII
The enzyme DNA polymerase extends the primers in PCR using the nucleotides provided in the reaction and the genomic DNA as a _________.
The enzyme extends primers using genomic DNA as a template.
Question 114 of 535
📘 CLASS XII
After transformation, untransformed recipient cells will _________ if spread on agar plates containing the antibiotic for which the recombinant DNA bears resistance.
Untransformed recipient cells will die on antibiotic-containing plates.
Question 115 of 535
📘 CLASS XII
In almost all recombinant technologies, the ultimate aim is to produce a desirable _________.
The ultimate aim in almost all recombinant technologies is to produce a desirable protein.
Question 116 of 535
📘 CLASS XII
Small volume cultures cannot yield appreciable quantities of products, necessitating the development of _________ for large-scale production.
Bioreactors are needed to process large volumes of culture.
Question 117 of 535
📘 CLASS XII
A stirred-tank bioreactor is usually cylindrical or with a _________ base to facilitate the mixing of the reactor contents.
A stirred-tank reactor is usually cylindrical or with a curved base.
Question 118 of 535
📘 CLASS XII
Besides an agitator system and an oxygen delivery system, a bioreactor must typically include a system for foam control, temperature control, pH control, and _________.
Bioreactors have sampling ports so that small volumes of the culture can be withdrawn periodically.
Question 119 of 535
📘 CLASS XII
The integration of natural science and organisms, cells, parts thereof, and molecular analogues for products and services is the definition given by the _________.
The EFB has given this definition of biotechnology.
Question 120 of 535
📘 CLASS XII
In the convention for naming restriction enzymes, EcoRI comes from *Escherichia coli* _________.