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535 questions in this collection · Page 13 of 54
Question 121 of 535
📘 CLASS XII
Biotechnology Principles And Processes
A palindrome in DNA is a sequence of base pairs that reads the same on the two strands when orientation of reading is kept the same, such as in the _________ direction.
The sequence reads the same on the two strands in the 5' $\rightarrow$ 3' direction.
Question 122 of 535
📘 CLASS XII
Biotechnology Principles And Processes
One of the enzymes isolated in 1963 responsible for restricting bacteriophage growth in *E. coli* added _________ groups to DNA.
One of the enzymes isolated in 1963 added methyl groups to DNA.
Question 123 of 535
📘 CLASS XII
Biotechnology Principles And Processes
The ability of restriction enzymes to leave sticky ends facilitates the action of the enzyme _________.
The stickiness of the ends facilitates the action of DNA ligase.
Question 124 of 535
📘 CLASS XII
Biotechnology Principles And Processes
Unless one cuts the vector and the source DNA with the _________ restriction enzyme, the recombinant vector molecule cannot normally be created.
The vector and source DNA must be cut by the same restriction enzyme.
Question 125 of 535
📘 CLASS XII
Biotechnology Principles And Processes
A high copy number plasmid vector would be preferred if one wants to recover _________ copies of the target DNA.
If one wants to recover many copies, the vector origin should support a high copy number.
Question 126 of 535
📘 CLASS XII
Biotechnology Principles And Processes
The normal *E. coli* cells do not carry resistance against any of the common antibiotics used as selectable markers, such as ampicillin, tetracycline, etc., making them useful for identifying _________.
The normal E. coli do not carry resistance, allowing selection of transformed cells.
Question 127 of 535
📘 CLASS XII
Biotechnology Principles And Processes
The letter ‘rop’ in the vector pBR322 codes for the proteins involved in the _________ of the plasmid.
rop codes for the proteins involved in the replication of the plasmid.
Question 128 of 535
📘 CLASS XII
Biotechnology Principles And Processes
In insertional inactivation using the $\beta$-galactosidase gene, the $\beta$-galactosidase gene gets inactivated due to insertion of _________.
The gene is inactivated because recombinant DNA is inserted within its coding sequence.
Question 129 of 535
📘 CLASS XII
Biotechnology Principles And Processes
To force bacteria to take up the plasmid, the bacterial cells must first be made ‘competent’ by treating them with a divalent cation such as _________.
The divalent cation used is typically calcium.
Question 130 of 535
📘 CLASS XII
Biotechnology Principles And Processes
The DNA coating micro-particles used in biolistics or gene gun methods for plants are made of gold or _________.
Cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA.