One method of introducing alien DNA into host cells uses ‘disarmed _________’ vectors, which when allowed to infect the cell, transfer the recombinant DNA.
The last method discussed uses ‘disarmed pathogen’ vectors.
Question 132 of 535
📘 CLASS XII
Nucleic acid is the genetic material of all organisms without exception; in the majority of organisms, this is _________.
In the majority of organisms, the genetic material is deoxyribonucleic acid or DNA.
Question 133 of 535
📘 CLASS XII
The two core techniques that enabled the birth of modern biotechnology are Genetic Engineering and _________ Engineering.
The two core techniques are Genetic engineering and Bioprocess engineering.
Question 134 of 535
📘 CLASS XII
Asexual reproduction primarily _________ the genetic information.
Asexual reproduction preserves the genetic information.
Question 135 of 535
📘 CLASS XII
One step in recombinant DNA technology is transferring the recombinant DNA into the host, followed by culturing the host cells in a medium at _________.
This step involves culturing the host cells in a medium at large scale.
Question 136 of 535
📘 CLASS XII
One of the three basic steps in genetically modifying an organism is the introduction of the identified DNA into the _________.
The second basic step is the introduction of the identified DNA into the host.
Question 137 of 535
📘 CLASS XII
Restriction enzyme EcoRI cuts the palindrome sequence: 5'——G A A T T C——3' and 3'——C T T A A G——5'. It cuts between G and A on both strands, leaving sticky ends with the sequence _________.
The cut is between G and A, leaving 5'-AATTC-3' overhang.
Question 138 of 535
📘 CLASS XII
When transferred into *Escherichia coli*, recombinant DNA uses the new host’s DNA polymerase enzyme to replicate and make multiple copies. This ability was called _________ of the antibiotic resistance gene in *E. coli*.
The ability to multiply copies was called cloning of the antibiotic resistance gene in E. coli.
Question 139 of 535
📘 CLASS XII
The restriction site present in the ampicillin resistance gene of pBR322 is not explicitly mentioned but typically used sites like Pst I or Pvu II are present. If we choose Pst I from the diagram, where does the insertion happen in the ampicillin gene?
Based on the figure provided (Figure 9.4), Pst I is shown within the ampR gene.
Question 140 of 535
📘 CLASS XII
The final step in recombinant DNA technology is the extraction of the desired product and subsequent _________.
The process includes extraction of the desired product (functional protein) and purification.
