If we link an alien piece of DNA with bacteriophage or plasmid DNA, we can multiply its numbers equal to the _________ of the vector.
The alien DNA will multiply its numbers equal to the copy number of the vector.
Question 152 of 535
📘 CLASS XII
If foreign DNA is ligated at the BamH I site of the tetracycline resistance gene in pBR322, the transformants growing on ampicillin containing medium are then transferred to a medium containing _________ to select recombinants.
The second plating step uses tetracycline to check for inactivation.
Question 153 of 535
📘 CLASS XII
Retroviruses have been disarmed and are now used to deliver desirable genes into _________ cells.
Retroviruses are used to deliver desirable genes into animal cells.
Question 154 of 535
📘 CLASS XII
Incubating competent cells with recombinant DNA on ice, followed by brief exposure to 42°C (heat shock), and then putting them back on ice, enables the bacteria to take up the _________.
This procedure enables the bacteria to take up the recombinant DNA.
Question 155 of 535
📘 CLASS XII
In PCR, the steps in each cycle are (i) Denaturation, (ii) Primer annealing, and (iii) _________ of primers.
The three steps are Denaturation, Primer annealing, and Extension of primers.
Question 156 of 535
📘 CLASS XII
The primary purpose of using a continuous culture system is that this method produces a larger biomass leading to _________ yields of desired protein.
Continuous culturing produces a larger biomass leading to higher yields.
Question 157 of 535
📘 CLASS XII
The stirred-tank bioreactor has an agitator system, an oxygen delivery system, a foam control system, a temperature control system, a pH control system, and _________.
The bioreactor includes sampling ports for periodic culture withdrawal.
Question 158 of 535
📘 CLASS XII
Bioreactors can be thought of as vessels in which raw materials are biologically converted into specific products using microbial plant, animal or _________ cells.
Bioreactors use microbial, plant, animal or human cells.
Question 159 of 535
📘 CLASS XII
The process of downstream processing involves separation and purification, followed by formulation with suitable preservatives, and thorough clinical trials, which is necessary for products like _________.
Clinical trials are required as in the case of drugs.
Question 160 of 535
📘 CLASS XII
The techniques of genetic engineering overcome the limitation of traditional hybridization procedures by allowing the introduction of only a set of desirable genes, contrasting with the tendency of traditional methods to lead to multiplication of _________ genes.
Traditional methods multiply undesirable genes along with desired ones, a limitation overcome by genetic engineering.
