The specific palindromic sequence which is recognized by EcoRI is:

NEET-(Sept)2020 Molecular-Basis-of-Inheritance [class-xii ]

• 5'-GAATTC-3'
  3'-CTTAAG-5'
• 5'-GGAACC-3'
  3'-CCTTGG-5'
• 5'-CTTAAG-3'
  3'-GAATTC-5'
• 5'-GGATCC-3'
  3'-CCTAGG-5'
Correct Option: A  [ 5'-GAATTC-3'
3'-CTTAAG-5' ]

Remark:

By which method was a new breed 'Hisardale' of sheep formed by using Bikaneri ewes and Marino rams?

NEET-(Sept)2020 Strategies-for-Enhancement-in-Food-Production [class-xii ]

• Out crossing
• Mutational breeding
• Cross breeding
• Inbreeding
Correct Option: C  [ Cross breeding ]

Remark:

Inbreeding depression is:

NEET-(Oct)2020 Strategies-for-Enhancement-in-Food-Production [class-xii ]

• Reduced motility and immunity due to close inbreeding
• Decreased productivity due to mating of superior male and inferior female
• Decrease in body mass of progeny due to continued close inbreeding
• Reduced fertility and productivity due to continued close inbreeding
Correct Option: D  [ Reduced fertility and productivity due to continued close inbreeding ]

Remark:

Choose the correct pair from the following:

NEET-(Sept)2020 Biotechnology-Principles-and-Processes [class-xii ]

• Ligases -

  Join the two DNA molecules

• Polymerases -

  Break the DNA into fragments

• Nucleases -

  Separate the two strands of DNA

• Exonucleases -

  Make cuts at specific positions within DNA

Correct Option: A  [
Ligases -

Join the two DNA molecules
]

Remark:

Identify the wrong statement with regard to Restriction Enzymes.

NEET-(Sept)2020 Biotechnology-Principles-and-Processes [class-xii ]

• Each restriction enzyme functions by inspecting the length of a DNA sequence.
• They cut the strand of DNA at palindromic sites.
• They are useful in genetic engineering.
• Sticky ends can be joined by using DNA ligases.
Correct Option: D  [ Sticky ends can be joined by using DNA ligases. ]

Remark:

Match the following columns and select the correct option.

NEET-(Sept)2020 Biotechnology-Principles-and-Processes [class-xii ]

• (A)-(IV), (B)-(I), (C)-(II), (D)-(III)
• (A)-(III), (B)-(II), (C)-(I), (D)-(IV)
• (A)-(II), (B)-(III), (C)-(IV), (D)-(I)
• (A)-(I), (B)-(II), (C)-(III), (D)-(IV)
Correct Option: A  [ (A)-(IV), (B)-(I), (C)-(II), (D)-(III) ]

Remark:

In gel electrophoresis, separated DNA fragments can be visualized with the help of:

NEET-(Sept)2020 Biotechnology-Principles-and-Processes [class-xii ]

• Acetocarmine in bright blue light
• Ethidium bromide in UV radiation
• Acetocarmine in UV radiation
• Ethidium bromide in infrared radiation
Correct Option: D  [ Ethidium bromide in infrared radiation ]

Remark:

Match the organism with its use in biotechnology.

NEET-(Sept)2020 Biotechnology-and-its-applications [class-xii ]

• (A)-(II), (B)-(IV), (C)-(III), (D)-(I)
• (A)-(IV), (B)-(III), (C)-(I), (D)-(II)
• (A)-(III), (B)-(II), (C)-(IV), (D)-(I)
• (A)-(III), (B)-(IV), (C)-(I), (D)-(II)
Correct Option: B  [ (A)-(IV), (B)-(III), (C)-(I), (D)-(II) ]

Remark:

Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to:

NEET-(Sept)2020 Biotechnology-and-its-applications [class-xii ]

• Insect pests
• Fungal diseases
• Plant nematodes
• Insect predators
Correct Option: A  [ Insect pests ]

Remark:

Which of the following statements is not correct?

NEET-(Sept)2020 Biotechnology-and-its-applications [class-xii ]

• In man insulin is synthesised as a proinsulin.
• The proinsulin has an extra peptide called C-peptide.
• The functional insulin has A and B chains linked together by hydrogen bonds.
• Genetically engineered insulin is produced in E-Coli.
Correct Option: C  [ The functional insulin has A and B chains linked together by hydrogen bonds. ]

Remark: