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NEET Botany PYQs
Class XI & Class XII
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🧬 Botany
📘 Class XI & XII
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408 questions in Botany
The specific palindromic sequence which is
recognized by EcoRI is:
A
5'-GAATTC-3'
B
5'-GGAACC-3'
C
5'-CTTAAG-3'
D
5'-GGATCC-3'
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By which method was a new breed 'Hisardale' of
sheep formed by using Bikaneri ewes and Marino
rams?
A
Out crossing
B
Mutational breeding
C
Cross breeding
D
Inbreeding
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Inbreeding depression is:
A
Reduced motility and immunity due to close
inbreeding
B
Decreased productivity due to mating of superior
male and inferior female
C
Decrease in body mass of progeny due to continued
close inbreeding
D
Reduced fertility and productivity due to continued
close inbreeding
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Choose the correct pair from the following:
A
Ligases -
Join the two DNA molecules
B
Polymerases -
Break the DNA into fragments
C
Nucleases -
Separate the two strands of DNA
D
Exonucleases -
Make cuts at specific positions within DNA
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Identify the wrong statement with regard to
Restriction Enzymes.
A
Each restriction enzyme functions by inspecting the length of a DNA sequence.
B
They cut the strand of DNA at palindromic sites.
C
They are useful in genetic engineering.
D
Sticky ends can be joined by using DNA ligases.
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Match the following columns and select the
correct option.
Column-I
Column-II
(A)Bt cotton
(I)Gene therapy
(B)Adenosine deaminase deficiency
(II)Cellular defence
(C)RNAi
(III)Detection of HIV infection
(D)PCR
(IV)Bacillus thuringiensis
A
(A)-(IV), (B)-(I), (C)-(II), (D)-(III)
B
(A)-(III), (B)-(II), (C)-(I), (D)-(IV)
C
(A)-(II), (B)-(III), (C)-(IV), (D)-(I)
D
(A)-(I), (B)-(II), (C)-(III), (D)-(IV)
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In gel electrophoresis, separated DNA fragments
can be visualized with the help of:
A
Acetocarmine in bright blue light
B
Ethidium bromide in UV radiation
C
Acetocarmine in UV radiation
D
Ethidium bromide in infrared radiation
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Explanation: In gel electrophoresis, DNA fragments are stained with ethidium bromide and then viewed under ultraviolet (UV) light. Ethidium bromide intercalates between DNA bases and fluoresces, making the DNA bands visible. This is described in NCERT as a standard method for visualization.
🧠Did You Know? Ethidium bromide emits orange fluorescence when bound to DNA and exposed to UV light, revealing band patterns that correspond to fragment sizes.
💡 NCERT Memory Line: DNA is stained with a DNA-specific dye (ethidium bromide) and visualized under UV light to see gel bands.
Match the organism with its use in biotechnology.
(A)Bacillus thuringiensis
(I)Cloning vector
(B)Thermus aquaticus
(II)Construction of first rDNA molecule
(C)Agrobacterium tumefaciens
(III)DNA polymerase
(D)Salmonella typhimurium
(IV)Cry proteins
A
(A)-(II), (B)-(IV), (C)-(III), (D)-(I)
B
(A)-(IV), (B)-(III), (C)-(I), (D)-(II)
C
(A)-(III), (B)-(II), (C)-(IV), (D)-(I)
D
(A)-(III), (B)-(IV), (C)-(I), (D)-(II)
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Bt cotton variety that was developed by the
introduction of toxin gene of Bacillus thuringiensis
(Bt) is resistant to:
A
Insect pests
B
Fungal diseases
C
Plant nematodes
D
Insect predators
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Which of the following statements is not correct?
A
In man insulin is synthesised as a proinsulin.
B
The proinsulin has an extra peptide called
C-peptide.
C
The functional insulin has A and B chains linked together by hydrogen bonds.
D
Genetically engineered insulin is produced in E-Coli.
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