A DNA palindrome reads the same on the two strands when the orientation of reading is kept the same, typically in the:
The palindrome in DNA is a sequence that reads same on the two strands when orientation of reading is kept the same (e.g., 5' $\rightarrow$ 3' direction).
Question 292 of 535
📘 CLASS XII
The stickiness of the ends produced by restriction enzymes is crucial because it facilitates the action of which enzyme?
The stickiness of the ends facilitates the action of the enzyme DNA ligase to join the fragments.
Question 293 of 535
📘 CLASS XII
The recombinant vector molecule cannot normally be created unless one cuts the vector and the source DNA with:
Normally, one must cut the vector and the source DNA with the same restriction enzyme.
Question 294 of 535
📘 CLASS XII
A high copy number plasmid vector would be preferred if the desired outcome is to recover:
If one wants to recover many copies, the vector origin should support high copy number.
Question 295 of 535
📘 CLASS XII
In selection using antibiotic resistance genes, the purpose of using normal *E. coli* cells (which lack resistance) is that they are useful for identifying:
Normal E. coli cells do not carry resistance, allowing selection of cells that have been successfully transformed (picked up the plasmid).
Question 296 of 535
📘 CLASS XII
In the vector pBR322, the letter ‘rop’ codes for proteins involved in the:
rop codes for the proteins involved in the replication of the plasmid.
Question 297 of 535
📘 CLASS XII
The inactivation of the $\beta$-galactosidase gene in chromogenic selection is caused by the insertion of:
The gene is inactivated because recombinant DNA (alien DNA) is inserted within its coding sequence.
Question 298 of 535
📘 CLASS XII
The bacterial cells must be made ‘competent’ before transformation by treating them with a divalent cation such as calcium, which primarily increases the efficiency with which DNA enters the bacteria through:
Calcium increases the efficiency with which DNA enters the bacterium through pores in its cell wall.
Question 299 of 535
📘 CLASS XII
The micro-particles used in biolistics (gene gun) methods for bombarding plant cells are coated with DNA and typically made of gold or:
Cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA.
Question 300 of 535
📘 CLASS XII
The last method mentioned for introducing alien DNA into host cells involves using ‘disarmed _________’ vectors, which infect the cell and transfer the recombinant DNA.
