The first instance of artificial recombinant DNA construction involved isolating a gene that conferred resistance to:
The construction involved linking a gene encoding antibiotic resistance.
Question 312 of 535
📘 CLASS XII
A plasmid acts as a vector to transfer alien DNA, analogous to how a mosquito acts as an insect vector to transfer the _________ parasite.
A mosquito acts as an insect vector to transfer the malarial parasite into the human body.
Question 313 of 535
📘 CLASS XII
Restriction endonucleases inspect the length of a DNA sequence to find their specific:
Restriction endonucleases function by ‘inspecting’ the length of a DNA sequence to find their specific recognition sequence.
Question 314 of 535
📘 CLASS XII
If an alien piece of DNA is linked with bacteriophage or plasmid DNA, its numbers will multiply equal to the _________ of the vector.
The numbers of the alien DNA will multiply equal to the copy number of the plasmid or bacteriophage.
Question 315 of 535
📘 CLASS XII
If foreign DNA is inserted into the *ampR* gene of pBR322, the transformants would lose ampicillin resistance. To select these recombinants from non-recombinants, what medium is used for the *first* plating step?
The transformants growing on ampicillin containing medium are selected first, as non-transformants die. Loss of ampicillin resistance distinguishes recombinants in the second step.
Question 316 of 535
📘 CLASS XII
The tumor inducing (Ti) plasmid of *Agrobacterium tumifaciens* has been modified into a cloning vector which is no longer:
The Ti plasmid has been modified into a cloning vector which is no more pathogenic to the plants.
Question 317 of 535
📘 CLASS XII
The heat shock method (ice $
ightarrow$ 42°C $
ightarrow$ ice) is used to enable bacteria to take up recombinant DNA because it makes the bacteria:
This procedure enables the bacteria to take up the recombinant DNA after being made ‘competent’ by divalent cations and temperature changes.
Question 318 of 535
📘 CLASS XII
The three steps in each cycle of Polymerase Chain Reaction (PCR) are (i) Denaturation, (ii) Primer annealing, and (iii):
Each PCR cycle has three steps: (i) Denaturation; (ii) Primer annealing; and (iii) Extension of primers.
Question 319 of 535
📘 CLASS XII
The continuous culture system is primarily used in bioreactors because it maintains cells in the log/exponential phase, which leads to:
Continuous culturing produces a larger biomass leading to higher yields of desired protein.
Question 320 of 535
📘 CLASS XII
Bioreactors are vessels in which raw materials are biologically converted into specific products using microbial, plant, animal or _________ cells.
Bioreactors use microbial, plant, animal or human cells.
