Question 21 of 39
Choose the correct pair from the following:
Explanation will be generated when opened.
Biotechnology Principles and Processes NEET PYQs
Class XII Biology • NCERT Based • NEET Practice
Question 22 of 39
Identify the wrong statement with regard to
Restriction Enzymes.
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Question 23 of 39
Match the following columns and select the
correct option.
| Column-I | Column-II |
|---|---|
| (A)Bt cotton | (I)Gene therapy |
| (B)Adenosine deaminase deficiency | (II)Cellular defence |
| (C)RNAi | (III)Detection of HIV infection |
| (D)PCR | (IV)Bacillus thuringiensis |
Explanation will be generated when opened.
Question 24 of 39
In gel electrophoresis, separated DNA fragments
can be visualized with the help of:
Explanation:
In gel electrophoresis, DNA fragments are stained with ethidium bromide and then viewed under ultraviolet (UV) light. Ethidium bromide intercalates between DNA bases and fluoresces, making the DNA bands visible. This is described in NCERT as a standard method for visualization.
🧠 Did You Know?Ethidium bromide emits orange fluorescence when bound to DNA and exposed to UV light, revealing band patterns that correspond to fragment sizes.
💡 NCERT Memory Line:DNA is stained with a DNA-specific dye (ethidium bromide) and visualized under UV light to see gel bands.
Question 25 of 39
Spooling is:
Explanation:
Spooling refers to gathering or collecting the isolated DNA strands after extraction, i.e., the DNA that has been purified is spooled around a rod or stick. This is the collection step of DNA isolation. Hence, option 4 is correct.
🧠 Did You Know?In labs, DNA can appear as a white, cottony mass that can be wound or spooled on a glass rod when it precipitates out of solution.
💡 NCERT Memory Line:Spooling = collection of isolated DNA during isolation procedures.
Question 26 of 39
The sequence that controls the copy number of the
linked DNA in the vector, is termed:
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Question 27 of 39
Following statements describe the characteristics of the enzyme Restriction endonuclease. Identify the
incorrect statement.
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Question 28 of 39
Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:
Explanation: NCERT Page No.195
Question 29 of 39
What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism?
A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism.
B. It may get integrated into the genome of the recipient.
C. It may multiply and be inherited along with the host DNA.
D. The alien piece of DNA is not an integral part of chromosome.
E. It shows ability to replicate.
Choose the correct answer from the options given below:
A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism.
B. It may get integrated into the genome of the recipient.
C. It may multiply and be inherited along with the host DNA.
D. The alien piece of DNA is not an integral part of chromosome.
E. It shows ability to replicate.
Choose the correct answer from the options given below:
Explanation: NCERT Page No.194, (Topic-11.1 PRINCIPLES OF BIOTECHNOLOGY)
Question 30 of 39
Which of the following statements about lac-operon is correct?
The regulatory gene (i) is constitutively expressed. Allolactose, not galactose, acts as the inducer by inactivating the repressor.