Upon exposure to UV radiation, DNA stained with ethidium bromide will show:

NEET-2023 [Biotechnology-Principles-and-Processes] [class-xii ]

• Bright orange colour
• Bright red colour
• Bright blue colour
• Bright yellow colour
Correct Option: A  [ Bright orange colour ]

Remark:

In gene gun method used to introduce alien DNA into host cells, microparticles of ______ metal are used.

NEET-2023 [Biotechnology-Principles-and-Processes] [class-xii ]

• Silver
• Copper
• Zinc
• Tungsten or gold
Correct Option: D  [ Tungsten or gold ]

Remark:

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:

NEET-2023 [Biotechnology-Principles-and-Processes] [class-xii ]

• Polysaccharides
• RNA
• DNA
• Histones
Correct Option: C  [ DNA ]

Remark:

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
A. Insertion of recombinant DNA into the host cell.
B. Cutting of DNA at specific location by restriction enzyme.
C. Isolation of desired DNA fragment.
D.Amplification of gene of interest using PCR
Choose the correct answer from the option given below:

NEET-2023 [Biotechnology-Principles-and-Processes] [class-xii ]

• B, D, A, C
• B, C, D, A
• C, A, B, D
• C, B, D, A
Correct Option: B  [ B, C, D, A ]

Remark:

Which of the following is not a cloning vector?

NEET-2023 [Biotechnology-Principles-and-Processes] [class-xii ]

• Probe
• BAC
• YAC
• pBR322
Correct Option: A  [ Probe ]

Remark:

Which one of the following statement is not true regarding gel electrophoresis technique?

NEET-2022 [Biotechnology-Principles-and-Processes] [class-xii ]

• The process of extraction of separated DNA strands from gel is called elution.
• The separated DNA fragments are stained by using ethidium bromide.
• The presence of chromogenic substrate gives blue coloured DNA bands on the gel.
• Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.
Correct Option: C  [ The presence of chromogenic substrate gives blue coloured DNA bands on the gel. ]

Remark:

Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): Polymerase chain reaction is used in DNA amplification.
Reason (R): The ampicillin resistant gene is used as a selectable marker to check transformation
In the light of the above statements, choose the correct answer from the options given below :

NEET-2022 [Biotechnology-Principles-and-Processes] [class-xii ]

• Both (A) and (R) are correct and (R) is the correct explanation of (A)
• Both (A) and (R) are correct but (R) is not the correct explanation of (A)
• (A) is correct but (R) is not correct
• (A) is not correct but (R) is correct
Correct Option: B  [ Both (A) and (R) are correct but (R) is not the correct explanation of (A) ]

Remark:

In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?

NEET-2022 [Biotechnology-Principles-and-Processes] [class-xii ]

• 5'GATACT3'; 3'CTATGA5'
• 5'GAATTC3'; 3'CTTAAG5'
• 5'CTCAGT3'; 3'GAGTCA5'
• 5'GTATTC3'; 3'CATAAG5'
Correct Option: B  [ 5'GAATTC3'; 3'CTTAAG5' ]

Remark:

Given below are two statements:
Statement I: Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence.
Statement II: Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site.
In the light of the above statements, choose the most appropriate answer from the options given below:

NEET-2022 [Biotechnology-Principles-and-Processes] [class-xii ]

• Both Statement I and Statement II are incorrect
• Statement I is correct but Statement II is incorrect
• Statement I is incorrect but Statement II is correct
• Both Statement I and Statement II are correct
Correct Option: D  [ Both Statement I and Statement II are correct ]

Remark:

Which of the following is not a desirable feature of a cloning vector?

NEET-2022 [Biotechnology-Principles-and-Processes] [class-xii ]

• Presence of a marker gene
• Presence of single restriction enzyme site
• Presence of two or more recognition sites
• Presence of origin of replication
Correct Option: C  [ Presence of two or more recognition sites ]

Remark: