Identify the wrong statement with regard to
Restriction Enzymes.
AI-generated explanation. Check against your textbook.
Explanation:
The wrong statement is option 1. Restriction enzymes do not inspect the length of a DNA sequence; they recognize and cut at specific short, usually palindromic, nucleotide sequences. The site is defined by its sequence, not by a general length check. Options 2, 3, and 4 are correct: restriction enzymes cut at palindromic sites; they are useful in genetic engineering; and DNA ligases can join sticky ends created by these enzymes.
🧠 Did You Know?
Restriction enzymes generate either sticky (overhang) or blunt ends, which are used to ligate DNA fragments in genetic engineering.
💡 NCERT Memory Line:
Restriction enzymes cut DNA at specific palindromic sequences, enabling precise gene manipulation in biotechnology.
In gel electrophoresis, separated DNA fragments
can be visualized with the help of:
AI-generated explanation. Check against your textbook.
Explanation:
In gel electrophoresis, DNA fragments are stained with ethidium bromide and then viewed under ultraviolet (UV) light. Ethidium bromide intercalates between DNA bases and fluoresces, making the DNA bands visible. This is described in NCERT as a standard method for visualization.
🧠 Did You Know?
Ethidium bromide emits orange fluorescence when bound to DNA and exposed to UV light, revealing band patterns that correspond to fragment sizes.
💡 NCERT Memory Line:
DNA is stained with a DNA-specific dye (ethidium bromide) and visualized under UV light to see gel bands.