Arrange the following steps of somatic hybridisation in the correct sequence:
(A) Digestion of cell walls
(B) Isolation of naked protoplasts
(C) Fusion of protoplasts to get hybrid protoplast
(D) Isolation of single cells from two different varieties of plants
(E) Growing of hybrid protoplast to form a new plant
Single cells are isolated first, cell walls are digested to obtain protoplasts, protoplasts are fused, and the hybrid protoplast is cultured to regenerate a new plant.
The toxin proteins isolated from Bacillus thuringiensis, coded by which of the following genes would control cotton bollworms and corn borer, respectively?
The cryIAc gene is effective against cotton bollworms, whereas the cryIAb gene is effective against corn borer.