Which one of the following techniques does not serve the purpose of early diagnosis of a disease for its early treatment?

NEET-2023 [Biotechnology-and-its-Applications] [class-xii ]

• Enzyme Linked Immuno-Sorbent Assay (ELISA) technique
• Recombinant DNA Technology
• Serum and Urine analysis
• Polymerase Chain Reaction (PCR) technique
Correct Option: C  [ Serum and Urine analysis ]

Remark:

Transposons can be used during which one of the following ?

NEET-2022 [Biotechnology-and-its-applications] [class-xii ]

• Polymerase Chain Reaction
• Gene Silencing
• Autoradiography
• Gene sequencing
Correct Option: B  [ Gene Silencing ]

Remark:

Statements related to human Insulin are given below.
Which statement(s) is/ are correct about genetically engineered Insulin?
A. Pro-hormone insulin contain extra stretch of C-peptide
B. A-peptide and B-peptide chains of insulin were produced separately in E.coli, extracted and combined by creating disulphide bond between them.
C. Insulin used for treating Diabetes was extracted from Cattles and Pigs.
D. Pro-hormone Insulin needs to be processed for converting into a mature and functional hormone.
E. Some patients develop allergic reactions to the foreign insulin. Choose the most appropriate answer from the options given below:

NEET-2022 [Biotechnology-and-its-Applications] [class-xii ]

• B only
• C and D only
• C, D and E only
• A, B and D only
Correct Option: A  [ B only ]

Remark:

When gene targetting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as:

NEET-2021 [Biotechnology-and-its-applications] [class-xii ]

• Biopiracy
• Gene therapy
• Molecular diagnosis
• Safety testing
Correct Option: B  [ Gene therapy ]

Remark:

Which of the following is not an application of PCR (Polymerase Chain Reaction)?

NEET-2021 [Biotechnology-and-its-applications] [class-xii ]

• Molecular diagnosis
• Gene amplification
• Purification of isolated protein
• Detection of gene mutation
Correct Option: C  [ Purification of isolated protein ]

Remark:

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

NEET-2021 [Biotechnology-and-its-applications] [class-xii ]

• Denaturation, Annealing, Extension
• Denaturation, Extension, Annealing
• Extension, Denaturation, Annealing
• Annealing, Denaturation, Extension
Correct Option: A  [ Denaturation, Annealing, Extension ]

Remark:

Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because:

NEET-2021 [Biotechnology-and-its-applications] [class-xii ]

• mutated gene partially appears on a photographic film.
• mutated gene completely and clearly appears on a photographic film.
• mutated gene does not appear on a photographic film as the probe has no complimentarity with it.
• mutated gene does not appear on photographic film as the probe has complimentarity with it.
Correct Option: C  [ mutated gene does not appear on a photographic film as the probe has no complimentarity with it. ]

Remark:

With regard to insulin choose correct options.
A. C-peptide is not present in mature insulin.
B. The insulin produced by rDNA technology has C-peptide.
C. The pro-insulin has C-peptide.
D. A-peptide and B-peptide of insulin are interconnected by disulphide bridges.
Choose the correct answer from the options given below.

NEET-2021 [Biotechnology-and-its-Applications] [class-xii ]

• B and D only
• B and C only
• A, C and D only
• A and D only
Correct Option: C  [ A, C and D only ]

Remark:

For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?

NEET-2021 [Biotechnology-and-its-Applications] [class-xii ]

• Western Blotting Technique
• Southern Blotting Technique
• ELISA Technique
• Hybridization Technique
Correct Option: C  [ ELISA Technique ]

Remark:

The Adenosine deaminase deficiency results into:

NEET-2021 [Biotechnology-and-its-Applications] [class-xii ]

• Dysfunction of Immune system
• Parkinson's disease
• Digestive disorder
• Addison's disease
Correct Option: A  [ Dysfunction of Immune system ]

Remark: